Lipocalin2 Protects Human Embryonic Kidney Cells against Cisplatin–Induced Genotoxicity
Authors
Abstract:
Cisplatin is one of the most useful chemotherapeutics which performs its cytotoxic effectvia accumulation of platinum resulting in oxidative stress, and destruction of cell DNA. Thiscould probably cause secondary cancers in healthy tissues. Lipocalin2 (Lcn2) is a protein whichits expression is increased in oxidative stresses. Therefore, the present study was performedto evaluate the protective effects of Lcn2 up-regulation on cisplatin genotoxicity. In order toup-regulate Lcn2 expression, HEK293 cells were transfected with pcDNA3.1-Lcn2 vector.Afterwards, stable cells consistently expressing Lcn2 were selected via screening with G418antibiotic. Next, overexpression of Lcn2 was evaluated by RT-PCR and ELISA, comparing tothe control non-transfected cells. Then, in order to evaluate the cytoprotective effects of Lcn2overexpression, transfected and non-transfected cells were subjected to cisplatin treatmentfollowed by MTT and alkaline Comet assays. RT-PCR and ELISA assays confirmed upregulationof Lcn2 by the stable cells. MTT assay of the Lcn2 over-expressing cells showedhigher IC50 values comparing to the non-transfected cells. Furthermore, the Comet assayconfirmed Lcn2 protective effects on the cisplatin (1 μg/mL) induced genotoxicity. In thepresent study, for the first time, we showed the protective effect of Lcn2 on cisplatin inducedgenotoxicity. Therefore, one of the probable mechanisms of Lcn2 cytoprotctive effects underoxidative stress conditions could be due to the prevention of genotoxicity. However, furtherevaluations in this regard must be considered.
similar resources
Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles
Traces of zinc oxide nanoparticles (ZnO NPs) used may be found in the liver and kidney. The aim of this study is to determine the optimal viability assay for using with ZnO NPs and to assess their toxicity to human hepatocyte (L02) and human embryonic kidney (HEK293) cells. Cellular morphology, mitochondrial function (MTT assay), and oxidative stress markers (malondialdehyde, glutathione (GSH) ...
full textThe phytoalexin, resveratrol ameliorates Ochratoxin A genotoxicity in Human Embryonic Kidney (HEK293) cells
ii Declaration iv Acknowledgements v Publications vii Presentations viii List of abbreviations ix List of figures xiii List of tables xvi
full textEffects of Biebersteinia multifida hydro-ethanol extract on proliferation and apoptosis of human prostate cancer and human embryonic kidney cells
Objective: Biebersteinia (Geraniaceae) has a history of use in traditional medicine in some countries including Iran. In the present study, cytotoxic and apoptogenic properties of hydro-ethanol extract of B. multifidi was investigated on human prostate cancer cell lines (PC3 and DU 145) and human embryonic kidney 293 (HEK293) cells. Materials and Methods: Cells were cultured in RPMI-1640 medium...
full textNimodipine Protects PC12 Cells against Oxygen-Glucose Deprivation
The protective effect of a L-type calcium channel blocker, nimodipine, on cell injury induced by oxygen-glucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were exposed to in-vitro oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10mM/L) in three different time schedules (pre-24h, pre-3h and concurrently). Cellular vi...
full textMy Resources
Journal title
volume 17 issue 1
pages 147- 154
publication date 2018-01-01
By following a journal you will be notified via email when a new issue of this journal is published.
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023